UDC 633.11:631.524.86:577.21
DOI 10.36461/NP.2026.77.1.013

IDENTIFICATION OF YELLOW RUST RESISTANCE GENES AND DEVELOPMENT OF SPECIFIC PRIMERS WITH “IN SILICO” ANALYSIS
J.K. Norbekov¹ (PhD), Head of Laboratory; N.N. Khusenov¹ (PhD), Head of Laboratory;
U.A. Boykobilov¹, Junior Researcher; A.Kh. Kushbakov^1,2, Master's Student;
R.I. Mukhammadaliev¹, Doctoral Student; D.Sh. Sultonov³, Master's Student;
N.R. Rakhmatova¹, Head of Laboratory
¹Center for Genomics and Bioinformatics, Academy of Sciences of the Republic of Uzbekistan,
Tashkent, Uzbekistan, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
²Tashkent State Agrarian University
³Bukhara State University

Yellow rust (Puccinia striiformis f. sp. tritici) is one of the most destructive fungal diseases of wheat, causing significant yield losses worldwide in Uzbekistan also. Nowadays, the development of resistant genes is considered a key strategy for ensuring sustainable wheat production. Current research deals with an RNA-seq dataset reflecting the interaction between wheat and the yellow rust pathogen, obtained from the NCBI database. The obtained data were examained with comprehensive “in silico” and bioinformatic analysis. Quality of RNA-seq results was assessed with FastQC, followed by filtering and trimming with the Fastp tool. High-quality data were compared to the wheat reference genome (IWGSC RefSeq v1.0) with HISAT2, and transcriptome assembly was carried out with StringTie. Differential gene expression analysis revealed a substantial number of genes responding to yellow rust infection across three bioprojects. In the first bioproject, 229 differentially expressed genes were identified; in the second – 202 genes; and in the third – 1005 genes. The results of the research provide a background for the development of novel DNA markers to be applied in wheat breeding programs aimed at creating yellow rust-resistant genes within a molecular-bioinformatic approach.
This research was carried out within the framework of the project AL-8523122265 “Development of a new wheat variety with high yield and quality characteristics, as well as resistant to yellow rust (Puccinia striiformis f.s. tritici)”.
Acknowledgments: The authors express their gratitude for the funding provided by the Agency for Innovative Development under the Ministry of Higher Education, Science and Innovations of the Republic of Uzbekistan, which supported the completion of this research under the project № AL-8523122265.
Keywords: wheat, yellow rust pathogen, RNA-seq, primer, in silico, transcriptome.

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